Terpene Esters from Natural Products: Synthesis and Evaluation of Cytotoxic Activity

ABSTRACT Natural steroids and triterpenes such as b-sitosterol, stigmasterol, lupeol, ursolic and betulinic acids were transformed into its hexanoic and oleic esters, to evaluate the influence of chemical modification towards the cytotoxic activities against tumor cells. The derivatives were evaluated against five tumor cell lines [OVCAR-8 (ovarian carcinoma); SF-295 (glioblastoma); HCT-116 (colon adenocarcinoma); HL-60 (leukemia); and PC-3 (prostate carcinoma)] and the results showed only betulinic acid hexyl ester exhibits cytotoxic potential activity.

Perezone, from the gorgonian Pseudopterogorgia rigida, induces oxidative stress in human leukemia cells

Abstract Four bisabolanes 1–4, including perezone (1) and triacetyl perezone (2), were isolated through a bioassay-guided fractionation of the extract obtained from the Caribbean gorgonian coral Pseudopterogorgia rigida collected during an expedition cruise to the Bahamas. All isolated compounds showed to be cytotoxic toward panel of four human tumor cell lines, as quantified by the MTT assay after 72 h incubation. Perezone (1), the most active one, was further analyzed, showing to be cytotoxic, but not selective, in a 12-cell line panel comprising tumor and non-tumor, as well as human and murine cells. Additionally, 1 was assayed for cytotoxicity against HL-60 leukemic cells. Pre-treatment with an acute free radical scavenger (L-NAC) before exposure of cells to perezone virtually eliminated the generation of intracellular ROS and lessened its severe cytotoxicity. The protective effect delivered by L-NAC evidences that the mechanism of perezone-induced cytotoxicity is partially associated to production of ROS and a consequent induction of oxidative stress.

Prevención de lesiones y retorno seguro al trabajo para pacientes con problemas lumbares que acuden al servicio de fisioterapia de la Policlínica Presidente Remón / Prevention of lesions and the secure return of patients with lumbar problems that sec the physical therapy services of the president Remon Policlinic to their work

Tiene como objetivo conocer el manejo más apropiado de los pacientes con diagnóstico de lesiones lumbares en el ámbito internacional, obtener datos sobre el particular a nivel nacional que permite conocer las diferencias para mejorarlas y proporcionar las conclusiones y recomendaciones que permitan divulgar los conocimientos sobre el problema

Prevención de lesiones y retorno seguro al trabajo para pacientes con problemas lumbares que acuden al servicio de fisioterapia en la policlínica Presidente Remón / Prevention of lesions and the secure return of patients with lumbar problems that seek the physical therapy services of the presidente Remón policlinic to their work

Se propone conocer el manejo más apropiado de los pacientes con diagnóstico de lesiones lumbares en el ámbito internacional, obtener datos sobre el particular a nivel nacional que permite conocer las diferencias para mejorarlas y proporcionar las conclusiones y recomendaciones que ayuden a divulgar los conocimientos sobre el problema